In-vitro oocyte maturation

In-vitro oocyte maturation -- primary oocytes recovered from small & growing follicles >/= 3 mm from ovaries of untreated women can be matured in vitro and then fertilized and developed in vitro and then transferred into the uterus

Goal: Immature oocytes matured in-vitro should have competent cytoplasmic & nuclear maturation and consequent embryo formed should have acceptable implantation rate

Limited practice due to scant knowledge of factors controlling developmental competence
Requires mimicking microendocrine environment of developing follicle
OR of –
Oocytes with or without exposure to GTs in vivo
Unstimulated polycystic ovaries
Ovariectomy specimen

Steps involved in In-vitro oocyte maturation

  1. Immature oocyte retrival (2-10 mm)
  2. In vitro maturation
  3. Sperm preparation
  4. Insemination or ICSI
  5. Endometrial preparation with HRT (Estrogens & progesterone)
  6. Embryo transfer

Future of In-vitro oocyte maturation

Eliminate medication to recruit cohort of follicles for OR
Reduce risk of OHSS
Reduce risk of long-term implications of ovarian carcinoma
Reduce cost of treatment
Assessment of oocyte quality by OR at diagnostic laproscopy
Pregnancy rate 2 % by Cha KY et al 1991
Till date only few pregnancies reported

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Dr. Patil’s Fertility and Endoscopy Clinic

Center for Assisted Reproductive Technology, Endoscopic Surgery and Andrology

No 1, Uma Admirality, First Floor, Bannerghatta Road, Bangalore - 560029
Ph: 080 - 41201357, 41462419 Mobile: 9945221622
E-Mail: drpatilsclinic@gmail.com

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