Cryopreservation

Surplus Embryos: While human embryo cryopreservation has become well-established technology in ART, it is yet to become fully, clear as to which stage preimplanattion embryos are best cryostored

Surplus Oocytes: Cryopreservation of human oocytes has been employed with little success in clinical practice, even though it may solve the legal & ethical problems linked with embryo freezing. Search for optimal protocol for oocyte cryopreservation remains elusive leading to low oocyte survival rates

Potential benefits

  1. Formation of donor “egg bank” to facilitate & lesson cost of oocyte donation
  2. Provision of egg cryostorage for women wishing to delay reproductive choice
  3. Cryopreservation of ovarian tissue from women about to undergo therapy deleterious to such tissue, which may threaten their reproductive health

Ovarian Tissue:

 

 

Testicular Tissue:

  • Success of infertility treatment Waltham 1st described in May 1996 use of frozen testicular tissue for achieving pregnancy
  • Azoospermic patients, use of single sperm for ICSI
  • Biopsy need not coincide with OR
  • Repeat procedure not required if diagnostic TB done and sperms isolated in biopsy (Gianaroli et al Italy)
  • Small piece of testicular tissue at open biopsy, frozen with same technique as sperms and stored in LN2 for future use.



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Dr. Patil’s Fertility and Endoscopy Clinic

Center for Assisted Reproductive Technology, Endoscopic Surgery and Andrology

No 1, Uma Admirality, First Floor, Bannerghatta Road, Bangalore - 560029
Ph: 080 - 41201357, 41462419 Mobile: 9945221622
E-Mail: drpatilsclinic@gmail.com

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